Familie Winchester
. Dean Winchester- Jensen Ackles
. Sam Winchester- Jared Padalecki
. Adam Milligan/Michael (Halbbruder von Sam und Dean)- Allison Hossack

Familie Harvelle
. Joanna Beth "Jo" Harvelle- Alona Tal
. Ellen Harvelle- Samantha Ferris

Engel
. Castiel- Misha Collins
.James Arthur-Taylor Lautner
. Michael- Matthew Cohen
. Uriel- Robert Wisdom
. Zachariah- Matthew Cohen
. Trickster/Erzengel Gabriel/Lok- Richard Speight junior
. Balthazar- Sebastian Roché
. Naomi- Amanda Tapping

Gefallene Engeln
. Luzifer- Mark Pellegrino/Jared Padalecki
. Anna Milton- Julie McNiven
.Diana Blair-

Geister
. Ash- Chad Lindberg

Fee
. Kattlea Prester- Kathie McGrath

Dämonen
. Ruby-Katie Cassidy/Genevieve Padalecki
. Crowley- Mark Sheppard
. Meg- Nicki Lynn Aycox/Rachel Miner
. Lilith- Rachel Pattee/Sierra McCormick/Katherine Boecher
. Alastair- Christopher Heyerdahl
. Der gelbäugige Dämon/Azazel- Fredric Lehne
. Eve (Die Mutter aller Monster)- Julia Maxwell/ Samantha Smith

Mensch
. Bela Talbot-Lauren Cohan- Lauren Cohan
. Chuck Shurley - Rob Benedict
. Ed Zeddmore- A. J. Buckley
. Harry Spengler- Travis Wester
. Jessica Moore- Adrianne Palicki
. FBI Agent Victor Henricksen- Charles Malik Whitfield
. Ronald Reznick- Chris Gauthier
. Andrew „Andy“ Gallagher - Gabriel Tigerman
. Ava Wilson- Katharine Isabelle
. Rufus Turner- Steven Williams
. Lisa Braeden- Cindy Sampson
. Sheriff Jody Mills- Kim Rhodes
. Becky Rosen- Emily Perkins
. Gwen Campbell- Jessica Heafey
. Christian Campbell- Corin Nemec
. Kevin Tran - Osric Chau
. Amelia- Liane Balaban

Leviathan
. Richard „Dick“ Roman- James Patrick Stuart

Sensenmann
. Tessa- Lindsey McKeon

Jäger
. Gordon Walker- Sterling K. Brown
. Garth Fitzgerald IV- DJ Qualls
. Robert „Bobby“ Singer- Jim Beaver

Vampir
. Lenore (Kuhblut)- Amber Benson
. Alpha Vampir- Rick Worthy
. Benny- Ty Olsson